Preparing replica film of specimen for electron microscopy

Details Preparing replica film of specimen for electron microscopy Preparing replica film of specimen for electron microscopy

1982-03-10

1984-05-08

Thurlow, Jeffery R.

Plastic and nonmetallic article shaping or treating: processes

Carbonizing to form article

Agglomeration or accretion

204165, 250311, 250326, 2504411, 2504421, 264 81, 264 83, 264220, 264221, 427 4, 427 39, 427 41, H05H 100

Patent

active

044473745

ABSTRACT:
A uniform thin replica film of a specimen for electron microscopy having a high resolution power such as approximately 1 .ANG. is presented. This uniform thin replica film is directly prepared on the specimen by a plasma polymerization technique by depositing organic monomer vapors on a specimen placed on a negative electrode in a high vacuum atmosphere. The deposition is carried out by applying a discharge voltage of from 0.5 to 3 K.V.D.C. between a pair of positive and negative electrodes for 1 to 15 minutes to effect a glow discharge under the conditions of a current density of the glow discharge of from 0.1 and 2 mA/cm.sup.2 and a gas pressure of from 1 to 10.sup.-2 Torr in the system.

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"Styrene Resin Cracking Method for Observing Biological Materials by Scanning Electron Microscopy", Journal of Electron Microscopy, Tanaka et al., vol. 23, No. 4, pp. 313-315 (1974).

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