Preparing membrane receptors from extracellular baculoviruses

Details Preparing membrane receptors from extracellular baculoviruses Preparing membrane receptors from extracellular baculoviruses

2006-04-25

2008-10-21

Ulm, John D (Department: 1649)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007210, C436S501000

Reexamination Certificate

active

07439030

ABSTRACT:
The present invention describes a method for producing membrane receptors based on a baculovirus/insect cell system. The invention further includes the receptors produced by the method described herein, a model for studying the properties of these membrane receptors and an assay to screen molecules that are active on these membrane receptors.

REFERENCES:
patent: WO 88 07082 (1988-09-01), None
patent: WO 96 09074 (1996-03-01), None
patent: WO 96 38575 (1996-12-01), None
Parker et al. Reconstitutively Active G Protein-coupled Receptors Purified from Baculovirus-infected Insect Cells. Jan. 5, 1991, J. Biol. Chem. 266(1):519-526.
Raivio, E., et al; “Expression of the Human Interleukin-2 Receptor Gamma Chain in Insect Cells Using a Baculovirus Expression Vector”;Scandinavian Journal of Immunology; 41, 338-342, (1995).
Loisel, T.P., et al; “Recovery of Homogeneous and Functional β2-adrenergic Receptors from Extracellular Baculovirus Particles”;Nature Biotechnology; 15(11), 1300-1304 (1997).
Parker, E.M., et al; “Reconstitutively Active G Protein-coupled Receptors Purified from Baculovirus-infected Insect Cells”;Journal of Biological Chemistry; vol. 266, No. 1, pp. 519-527, Jan. 5, 1991.

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