Preparing membrane receptors from extracellular baciloviruses

Details Preparing membrane receptors from extracellular baciloviruses Preparing membrane receptors from extracellular baciloviruses

2006-07-18

2006-07-18

Ulm, John (Department: 1649)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S005000, C435S384000

Reexamination Certificate

active

07078189

ABSTRACT:
Method of producing a recombinant membrane receptor protein by introducing a baculovirus expression vector encoding the membrane receptor protein into a host cell, culturing the resultant host cell for a sufficient time to permit expression of the membrane receptor protein and baculovirus viral particles and separating the cells from the viral particles. The baculovirus viral particles are then recovered.

REFERENCES:
patent: WO 88 07082 (1988-09-01), None
patent: WO 96 09074 (1996-03-01), None
patent: WO 96 38575 (1996-12-01), None
Parker et al, Reconstitutively Active G Protein-coupled Receptors Purified from Baculovirus-infected Insect Cells. J. Biol. Chem. 266(1):519-526, Jan. 1991.
Raivio, E., et al; “Expression of the Human Interleukin-2 Receptor Gamma Chain in Insect Cells Using a Baculovirus Expression Vector”;Scandianavian Journal of Immunology; 41, 338-342, (1995).
Loisel, T.P., et al; “Recovery of homogeneous and Functional β2-adrenergic Receptors from Extracellular Baculovirus Particles”;Nature Biotechnology; 15(11), 1300-1304 (1997).

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