Preparative two dimensional gel electrophoresis system

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

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204462, 204465, 204610, 204613, 204615, G01N 2726, G01N 27447

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active

055341217

ABSTRACT:
A preparative two dimensional gel electrophoresis system which serves as a single procedure for separation and isolation of preparative amounts of proteins from complex biological preparations. The system includes sized-up isoelectric focusing tube gels and slab gel molds which allow for sample loads of between about 0.5 and 2 mg or greater. Increased protein loads, resolution and electrotransfer allow for subsequent sequencing of separated proteins by conventional methods.

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Two-Dimensional Gel Electrophoresis by D. Rickwood et al. pp. 234-272.
Bio-Rad Life Science Research Products 1995 p. 164.

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