Preparative electrophoresis device and method

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, G01N 2726, G01N 27447

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active

052845591

ABSTRACT:
A preparative electrophoresis device having a pair of spaced apart plates, a pre-cast electrophoretic gel between the plates, and a semipermeable membrane sealably connected to ends of the plates to define with the plates a collection channel for receiving molecules that have traveled through the gel. Also disclosed are close spacing (less than 2.0 mm) of the plates, an inlet tube and an outlet tube connected to the two plates and communicating with opposite ends of the collection channel, an elongated divider between the plates to divide the gel into a purification region and an indicating region that is not as wide as the purification region and receives marker molecules of known molecular weight to indicate the location in the indicating region of molecules of interest, and pumping electrophoretic buffer into the collection channel at the same rate that it is pumped out.

REFERENCES:
patent: 3533933 (1970-10-01), Strauch
patent: 3697406 (1972-10-01), Svendson
patent: 4111785 (1978-09-01), Roskam
patent: 4707233 (1987-11-01), Margolis
patent: 4877510 (1989-10-01), Chen
patent: 5039386 (1991-08-01), Margolis
Bio-Rad Model 491 Prep Cell Instruction Manual.
Applied Biosystmes Model 230A HPEC System, High Performance electrphoresis brochure.
Hoeffer, SE 6101 Preparative Electrophoresis Accessory Kit Instructions.
Novel Experimental Technology, "A Solid System . . . You Can Jump Right Into" brochure.
Cavinato, A. G., "An Non-Denaturing Gel Electrophoresis System for the Purification of Membrane Bound Proteins", Preparative Biochemistry, pp. 205-216 (1988).
Sheer, D. G., "The Use of Micropreparative Electrophoresis of Protein/Peptide Isolations for Primary Structure Determinations", Product Application Focus, vol. 9, No. 4, pp. 486-495 (1990).
Bio-Rad Mini-Protean (TM) II Dual Slab Cell Instruction Manual.
Bio-Rad Model 491 Prep Cell Continuous Elution Electrophoresis SDS-PAGE brochure.
Bio-Rad Model A Modular Electrophoresis System for SDS-PAGE, 2-D, Blotting, and Electro-Elution, "Every Mini is Fast. Only One is Versatile." brochure.
A. Lemay "Separation of the Two High Molecular Weight Spectrin Bands by a Newly Designed Preparative Slab Electrophoresis Technique" Analytical Biochemistry 92 (1979) 130-135.
Chrambach, "The Practice of Quantitative Gel Electrophoresis", Advance Methods in the Biological Sciences, Chapter 4, pp. 19-62 and Chapter 10, pp. 170-185 (1985).
Speicher, D. W., "Microsequencing with PVDF Membranes: Efficient Electroblotting, Direct Protein Adsorption and Sequencer Program Modifications", Techniques in Protein Chemistry, pp. 24-35.
Horuk, R., "Preparative Polyacrylamide Gel Electrophoresis of Proteins", Advances in Electrophoresis, vol. 1, pp. 362-379.
Carpenter, H. P. et al., "A Device for Preparative Elution Electrophoresis Using a Polyacrylamide Gel Slab", Electrophoresis, vol. 7, pp. 221-226 (1986).
Groschup, M. H., et al., "A Convenient Gel Holder for Preparative Electrophoretic Separation of Aggregated Bacterial Proteins", Electrophoresis, vol. 12, pp. 90-91 (1991).
Hjerten, S., "Apparatus for Large-Scale Preparative Polyacrylamide Gel Electrophoresis", Analytical Biochemistry, vol. 27, pp. 108-129 (1969).
Hunkapiller, M. W., "Isolation of Microgram Quantities of Proteins from Polyacrylamide Gels for Amino Acid Sequence Analysis", Electroelution of Proteins from SDS Gels, vol. 19 pp. 227-236 (1983).
Laemmli, U. K., "Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4", Nature, vol. 227, pp. 680-685 (1970).
Schagger, H., "Tricine-Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis for the Separation of Proteins in the Range from 1 to 100 kDa", Analytic Biochemistry 166, pp. 368-379 (1987).

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