Preparative electrofocusing in flat bed granulated polysaccharid

Details Preparative electrofocusing in flat bed granulated polysaccharid Preparative electrofocusing in flat bed granulated polysaccharid

1979-07-18

1981-07-21

Prescott, Arthur C.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204180R, 204299R, 23230B, 424 12, G01N 2726

Patent

active

042797241

ABSTRACT:
Methods are described for the generation of natural pH gradients in layers of granulated gels using mixtures of amphoteric or amphoteric non-amphoteric buffer reagents. Because of the favorable characteristics of these flat bed systems, high voltage gradients (20-50 V/cm) can be used for the electrofocusing experiments. Linear or step pH gradients can be formed depending on the choice of buffer system used. Buffer systems, suitable for the preparative electrofocusing of proteins over the pI ranges of 4.0-6.0, 4.5-8.0, 5.0-8.5 and 6.0-8.5 are described.

REFERENCES:
patent: 4139440 (1979-02-01), Chrambach et al.

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