Preparations for use in solid phase immunoassays comprising mono

Details Preparations for use in solid phase immunoassays comprising mono Preparations for use in solid phase immunoassays comprising mono

1985-09-09

1988-02-23

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

424 11, 4351722, 435174, 43524027, 436519, 436547, 436548, 436804, 436535, 935110, G01N 3353, G01N 33558, C12N 1500

Patent

active

047270233

ABSTRACT:
A reagent for use in solid phase immunoassay diagnostics comprises a matrix of non-active hybridoma cells embedded with its self-produced, covalently bound, actively presented monoclonal antibodies.
The solid phase reagent according to the invention is prepared by incubating in vitro a culture medium containing active hybridoma cells capable of producing monoclonal antibodies, allowing the formation of antibodies to proceed, separating and washing said cells, resuspending the cells in a buffer solution, adding to the resulting suspension an inactivator substance capable of converting active hybridoma cells into the non-active state.

REFERENCES:
patent: 4409331 (1983-10-01), Lim
Taber's Cyclopedic Med. Dictionary (1973), Davis Co., Phila., Pa., p. F-25.

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