Preparation of water-insoluble enzymes

Details Preparation of water-insoluble enzymes Preparation of water-insoluble enzymes

1974-04-10

1976-06-08

Naff, David M.

Textiles: fiber preparation

195 63, 195DIG11, 20415916, 20415922, C07G 702

Patent

active

039620387

ABSTRACT:
Water-insoluble enzymes are prepared by mixing an enzyme with at least one water soluble monomer selected from the group consisting of acrylamide, bisacrylamide, acrylic acid, sodium acrylate, potassium acrylate and calcium acrylate, freezing the resulting mixture at a temperature below about -5.degree.C and irradiating the frozen mixture with gamma rays or x-rays under aerobic conditions to polymerize said monomer and entrap the enzyme in the resulting polymer material. The frozen irradiated polymer material can be thawed at room temperature or can be lyophilized to produce a spongelike, membrane form that maintains its structure when immersed in water. Before freezing the enzyme and monomer mixture, an organic substance can be incorporated which promotes polymerization yield and protects the enzyme from radiation inactivation.

REFERENCES:
patent: 3081244 (1963-03-01), Campanile
patent: 3137643 (1964-06-01), Bell et al.
patent: 3770588 (1973-11-01), Forgione
Hicks, et al., The Preparation and Characterization of Lyophilized Polyacrylamide Enzyme Gels for Chemical Analysis, Analytical Chemistry, vol. 38, No. 6, 1966 (pp. 726-730).

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