Preparation of water-insoluble enzyme compositions

Details Preparation of water-insoluble enzyme compositions Preparation of water-insoluble enzyme compositions

1978-05-31

1980-10-28

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

C07G 702

Patent

active

042308038

ABSTRACT:
Enzymes are covalently bonded to porous inorganic supports by a process wherein maximum activity of enzyme in the water-insoluble enzyme composition is achieved with the lowest possible amount of enzyme. The process involves selecting an inorganic carrier having a most frequent pore-diameter that results in highest enzymatic activity possible per weight unit of the insoluble enzyme composition at the given enzyme concentration within the insoluble enzyme composition, and contacting the selected inorganic carrier with an enzyme solution containing an amount of enzyme which is sufficiently low so that the enzyme in the insoluble enzyme composition has a specific activity which is substantially the same as that of the enzyme in solution in the free state.

REFERENCES:
patent: 3519538 (1970-07-01), Messing et al.
patent: 3850751 (1974-11-01), Messing
Messing, R. A., Immobilized Enzymes for Industrial Reactors, Academic Press, N.Y. 1975, (pp. 71-78, 104, 108 and 109).

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