Preparation of vanillin from microbial transformation media...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S252100

Reexamination Certificate

active

07981646

ABSTRACT:
Crude solid vanillin-containing material is precipitated from a solution obtained by biotransformation, and purified by a process comprising contacting it with a purification fluid selected from (a) a liquefied gas whose pressure exceeds its critical pressure and whose temperature is below its critical temperature; (b) a supercritical fluid; (c) a gas. The fluid is preferably liquid carbon dioxide. The temperature is maintained below 25°. The product may be further purified by treatment with CO2in a fluid bed drier. The crude material is preferably one precipitated from a solution resulting from biotransformation of ferulic acid. A new strain ofAmycolatopsiscapable of generating high concentrations of vanillin with minimal odoriferous by-products (e.g. guaiacol) is also disclosed.

REFERENCES:
patent: 4474994 (1984-10-01), Makin
patent: 4847422 (1989-07-01), Klemola et al.
patent: 6133003 (2000-10-01), Hopp et al.
patent: 6235507 (2001-05-01), Muheim et al.
patent: 0 885 968 (1998-12-01), None
patent: WO 00/50622 (2000-08-01), None

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