Preparation of uniformly sized liposomes encapsulating an aqueou

Details Preparation of uniformly sized liposomes encapsulating an aqueou Preparation of uniformly sized liposomes encapsulating an aqueou

1989-01-19

1991-05-21

Naff, David M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

264 41, 424450, 435177, 435182, 436829, G01N 33544, B01J 1302, A61K 3722, C12N 1102

Patent

active

050175017

ABSTRACT:
A dispersion of a uniformly sized population of multilamellar lipid vesicles (liposomes) encapsulating an aqueous liquid is prepared by forming a dried film of lipids on the walls of a vessel, contacting the film with an aqueous liquid in the presence of spherical contact masses such as glass beads and agitating. The liposomes have mean diameters in the range of about 150 to about 3000 nanometers and the contact masses have mean diameters of about 50 to 3,000 microns. The aqueous liquid encapsulated may contain enzymes, drugs or marker substances such as colorimetric or fluorescent dyes. A member of a immunological binding pair may be associated with the surfaces of the liposomes for carrying out immunoassays. This method allows for the use of small quantities of marker and lipid, leaves no residual solvents, allows for contact with only glass surfaces and involves no transfer of liposome preparations from lipid film drying vessels to sizing apparatus.

REFERENCES:
patent: 3887698 (1975-06-01), McConneil et al.
patent: 4224179 (1980-09-01), Schneider
patent: 4342826 (1982-08-01), Cole
patent: 4485054 (1984-11-01), Mezei et al.
Bangham, A. D., et al., J. Mol. Biol., 13:238-252 (1965).
Deamer, D. W. and Uster, P. S., "Liposomes", Ch. 1, Liposome Preparation: Methods and Mechanisms; and Marc J. Ostro (ed.), M. Dekker, Inc., pp. 27-51, New York, NY (1983).
Juliano, R. L. and Stamp, D., Bioch. Biophys. Res. Communications, 63:651-658 (1975).
Papahadjopoulos, D. and Miller, N., Biochimica et Biophysica Acta, 135:624-638 (1967).
Richards, R. L., et al., Biochimica et Biophysica Acta, 855:223-230 (1986).
Scherphof, G. L., et al., Biochemical Society Transactions, 15:345-348 (1987).
Scherphof, G. L., et al., Liposome Technology, vol. III, pp. 205-224, Edited by G. Gregnodia, CRC Press Boca Raton, Fla. (1984).
Szoka, F., Ann. Rev. Biophys. Bioeng., 9:467-508 (1980).
Szoka, F., et al., Proc. Nat'l. Acad. Sci. (USA), 75:4194-4198 (1978).
Wong, M., et al., Biochemistry, 21:4133-4139 (1982).

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