Preparation of uniform droplets by using gas pressure to force l

Details Preparation of uniform droplets by using gas pressure to force l Preparation of uniform droplets by using gas pressure to force l

1987-12-08

1995-08-15

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

264 5, 264 14, 435182, 43524022, 4352404, 435288, C12N 1110, C12N 1104, C12M 140, B29B 900

Patent

active

054418780

ABSTRACT:
An apparatus and method for the formation of droplets of uniform size on a laboratory scale are described. A syringe having a plunger and a needle with an orifice at the tip corresponding to a 12-30 gauge needle is inserted into a block member having a cavity such that the needle of the syringe extends through an opening in the bottom of the block member. The block member has a gas inlet into a side of the cavity for flowing of gas pass the orifice of the needle. The block member is mounted in a support housing such that the syringe is in a vertical position. To form droplets, pressure is applied to the plunger, preferably by a piston under gas pressure, to force liquid from the needle tip orifice to form droplets and flowing gas pass the tip to detach droplets of a desired size. In a preferred embodiment, the liquid is a suspension containing living cells and alginate, and droplets formed are solidified in a solidifying medium such as a calcium chloride solution to obtain microcapsules containing living cells.

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