Preparation of trichloro-s-triazine activated supports for coupl

Details Preparation of trichloro-s-triazine activated supports for coupl Preparation of trichloro-s-triazine activated supports for coupl

1978-02-09

1980-10-21

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

260 6, 435178, 435180, 435181, 435815, 544217, C07G 700, C07G 702

Patent

active

042295378

ABSTRACT:
Proteins and non-protein affinity ligands are covalently bonded to trichloro-s-triazine activated supports. The activated support is prepared by reacting a water-free insoluble solid support with trichloro-s-triazine in a non-aqueous medium and neutralizing HCl generated during the reaction with a tertiary amine which does not form an insoluble complex with the trichloro-s-triazine.

REFERENCES:
patent: 3619371 (1971-11-01), Crook et al.
patent: 3674767 (1972-07-01), Lilly et al.
patent: 3788948 (1974-01-01), Kagedal et al.
patent: 3824150 (1974-07-01), Lilly et al.
patent: 3876501 (1975-04-01), Hanushewsky
patent: 4007089 (1977-02-01), Smith
patent: 4119494 (1978-10-01), Durand et al.
Kay et al., The Chemical Attachment of Chymotrypsin to Water-Insoluble Polymers Using 2-Amino-4,6-Dichloro-s-Triazine, Biochimicz et Biophysicz Acta, vol. 198, 1970 (pp. 276-285).
Abuchowski et al., Alteration of Immunological Properties of Bovine Serum Albumin by Covalent Attachment of Polyethylene Glycol, J. Biol. Chem., vol. 252, No. 11, 1977 (pp. 3578-3581).

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