Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-02-09
1996-07-16
Naff, David M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
424422, 424423, 4352402, 435 11, C12N 500, C12M 304, A61F 1300, A61F 200
Patent
active
055366562
ABSTRACT:
Tissue equivalents are produced by applying a solution of collagen without cells to a permeable membrane, gelling to produce a collagen gel on the membrane, applying a mixture of collagen and a contractile agent to the collagen gel, gelling the mixture and allowing the resultant gel to undergo radial contraction which is controlled by the collagen gel on the membrane. A suitable contractile agent is fibroblast cells. A skin tissue equivalent is produced by disposing human epidermal cells on the contracted collagen gel and allowing epidermalization to occur. A nutrient medium can be supplied to the cells. An absorbent member may be disposed adjacent the permeable membrane opposite the collagen gel on the membrane. The permeable membrane can be the bottom of an inner well disposed in an outer well for containing a nutrient medium in contact with the membrane. The nutrient medium may be contained in the outer well in an agarose gel in contact with the membrane.
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Bell Eugene
Cavallaro John
Kagan David T.
Kemp Paul
Mason Valerie
Buckley Linda M.
Conlin David G.
Naff David M.
Organogenesis Inc.
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