Preparation of thaumatin sweeteners

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 697, 435 701, 4352543, 4352545, 4353201, 536 231, 536 234, 536 2374, 935 22, C12P 2106, C12P 2104, C07H 2102, C07H 2104

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059324384

ABSTRACT:
Thaumatins, protein sweeteners, are obtained through the expression of artificial, synthetic and substantially optimized genes, preferably in filamentous fungi such as Penicillium roquefortii, Aspergillus niger and Aspergillus niger var. awamori. Preparing substantially optimized artificial genes allows for high protein expression, making the process useful for industrial production of this valuable sweetener. Thaumatins may be obtained extracellularly and intracellularly. Intracellular production provides thaumatin-containing fungi that can be used per se in animal feed without prior separation of the fungal mycelium.

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