Preparation of synthetic frythrocytes

Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis

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514 6, A61K 3514, A61K 3518

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045321302

ABSTRACT:
A sterile preparation of synthetic erythrocytes consisting of hemoglobin fraction encapsulated within water-immiscible amphiphylic membranes provides a total hemoglobin of at least about 12 gm percent at a hematocrit of 50%. A lipid composition is prepared and dispersed by agitation in a sterile, stroma-free 30-45 gram percent hemoglobin fraction. The dispersion is pressurized to between about 400 to about 900 kg/cm.sup.2, and the pressure is substantially instantaneously released by passing the mixture to a lower pressure region through an orifice having an area of between about 0.1 and about 10.0 mm.sup.2, thereby forming the synthetic erythrocytes. The preparation is filtered through a filter which passes particles having a diameter less than about 0.22 micron to eliminate any monocellular organisms that may be present and to eliminate larger synthetic erythrocytes.
The synthetic erythrocyte preparation, if dried under vacuum to remove the major portion of the water from the encapsulated hemoglobin fraction, substantially transforms the erythrocytes but retains the integrity of the encapsulating lipid composition membranes. In the dried form, the sterile erythrocytes are storable for extended periods.

REFERENCES:
Djordjevich-Dissertation (1979), University Microfilms Internat. (1981), 7925989.
Djordjevich-Dissertation Abst. Int. B, (1979), vol. 40, No. 5, p. 2281.
Remington's Pharmaceutical Sciences 16th edition, (1980), pp. 1454-1457.
Szoka-Ann. Rev. Biophys. Bioeng., vol. 9, (1980), pp. 467, 485 & 486.

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