Preparation of support matrices for immobilized enzymes

Details Preparation of support matrices for immobilized enzymes Preparation of support matrices for immobilized enzymes

1979-11-07

1981-02-10

Marquis, Melvyn I.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

260 4213, 525374, 525376, 525383, 525386, C08K 300

Patent

active

042500800

ABSTRACT:
Support matrices for immobilized enzymes may be prepared by treating a solid porous, inorganic, water-insoluble support such as an alumina with a prepolymerized polymeric compound such as polystyrene. After treating the solid support the resultant organic-inorganic composite may then be derivatized, one example of derivatization being nitration of the composite with nitric acid and reducing the nitro-substituted composite to form an aminopolystyrene alumina composite. This composite is then treated with a bifunctional monomer to form a copolymeric material which is substantially entrapped in the pores of the solid support, said copolymeric material containing functionalized pendent groups to which an enzyme may be coupled to form an immobilized enzyme conjugate.

REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 3783101 (1974-01-01), Tomb et al.
patent: 3821084 (1974-06-01), Matthews et al.

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