Preparation of support matrices for immobilized enzymes

Chemistry of carbon compounds – Miscellaneous organic carbon compounds

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435180, 260 4243, 260 4254, 525344, 525374, 525384, C08K 900

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active

042183634

ABSTRACT:
Support matrices for immobilized enzymes may be prepared by treating a solid porous, inorganic, water-insoluble support such as an alumina with a prepolymerized polymeric compound. After treating the solid support the resultant organic-inorganic composite may then be derivatized, one example of derivatization being nitration of the composite with nitric acid and reducing the nitro-substituted composite to form an aminopolystyrene alumina composite. This composite is then treated with a bifunctional monomer to form a copolymeric which is substantially entrapped in the pores of the solid support, said copolymeric material containing functionalized pendent groups to which an enzyme may be coupled to form an immobilized enzyme conjugate.

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patent: 3654003 (1972-04-01), Grossman
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patent: 3821083 (1974-06-01), VanLeemputten et al.

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