Preparation of stable formulations of lipid-nucleic acid...

Details Preparation of stable formulations of lipid-nucleic acid... Preparation of stable formulations of lipid-nucleic acid...

2005-04-04

2008-12-09

Ketter, James S (Department: 1636)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C424S450000, C536S023100

Reexamination Certificate

active

07462603

ABSTRACT:
The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab′ fragment attached to a hydrophilic polymer.

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Zelphati, Olivier et al.; “Mechanism of oligonucleotide release from cationic liposomes”; 1996,PNAS, vol. 93, pp. 11493-11498.

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