Preparation of stabilized carrier-bound proteins

Details Preparation of stabilized carrier-bound proteins Preparation of stabilized carrier-bound proteins

1978-07-13

1981-01-27

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 44, 435180, 435188, 435230, 260112R, C12N 1110, C12N 1108, C12N 996

Patent

active

042476437

ABSTRACT:
Stabilized carrier-bound protein is prepared by reacting a protein in aqueous solution with a mercapto group free, water insoluble carrier which contains binding groups which are reactive with the said protein and reacting the resultant protein-containing mercapto group-free carrier with hydrogen sulfide or with a compound having a molecular weight of less than 5000, and which contains at least two mercapto groups, or at least one mercapto group and at least one hydroxy- group or primary or secondary amino group.

REFERENCES:
patent: 3645852 (1972-02-01), Axon et al.
patent: 3853708 (1974-12-01), Porath et al.
patent: 4008126 (1977-02-01), Keyes
Cleland, W. W. Dithiothreitol, A New Protective Reagent for SH Groups, Biochemistry, vol. 3, No. 4 1964, pp. (480-482).
Konigsberg, W. Reduction of Disulfide Bonds in Proteins with Dithiothreitol Methods in Enzymology, vol. XXXV, 1972 (pp. 185-188).

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