Preparation of sized populations of liposomes

Details Preparation of sized populations of liposomes Preparation of sized populations of liposomes

1987-11-25

1989-10-10

Dixon, Jr., William R.

Plastic and nonmetallic article shaping or treating: processes

Encapsulating normally liquid material

Liquid encapsulation utilizing an emulsion or dispersion to...

264 41, 424450, 436829, A61K 950, B01J 1302

Patent

active

048730351

ABSTRACT:
The present invention provides novel methods for generating aqueous liquid encapsulating multi-lamellar lipid vesicles (liposomes) wherein one or more lipids, or lipid conjugates, and an aqueous liquid to be encapsulated, are agitated in a vessel in the presence of spherical contact masses having a mean diameter of less than 3,000 microns, the preferred size range being 50-100 microns in diameter, and resulting in a substantially homogeneous population of vesicles having diameters in the range of about 150 to about 3,000 nanometers. The methods allow for the use of small quantities of marker and lipid, leave no residual solvents, allow for contact only with glass surfaces, and involve no transfer of liposome preparations from lipid film drying vessels to sizing apparatus.

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