Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1997-11-19
2000-03-14
Naff, David M.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435325, 435347, 435404, 435948, C12N 500, C12N 508
Patent
active
060371749
ABSTRACT:
Provided are serum-free, animal protein-free media formulations to be used in conjunction with hematopoietic growth factors for the in vitro growth of human neutrophil and megakaryocyte precursors. The medium contains a base medium, corticosteroid, transferrin, insulin, cholesterol, ethanolamine, and human albumin. Also provided are methods for preparing serum-free, animal protein-free suspensions of human hematopoietic precursor cells wherein the cellular component contains at least about 16% neutrophil precursors and at least about 1% megakaryocyte precursors. Serum-free, animal protein-free suspensions of human hematopoietic cells are provided wherein the cellular component comprises at least about 30%, preferably greater than 60% neutrophil precursors. The neutrophil precursors are comprised of blast cells, promyclocytes, neutrophilic myelocytes, and neutrophilic metamyelocytes. Serum-free, animal protein-free cells suspensions are provided wherein the cellular component comprises at least about 3%, preferably greater than 8% megakaryocyte precursors. Also provided are serum-free, animal protein free cell suspensions wherein the cellular component comprises colony-forming cells and cluster-forming cells.
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Bender James G.
Epps Dennis Van
Loudovaris Maureen F.
Maciukas Susan M.
Qiao Xiaoying
Naff David M.
Nexell Therapeutics Inc.
Ware Deborah K.
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