Preparation of secretin

Details Preparation of secretin Preparation of secretin

1984-08-08

1987-12-08

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 68, 435 69, 435 71, 4351723, 435227, 435228, 935 14, 935 51, 935 72, C12P 2102, C12P 2106, C12N 1500, C12N 978

Patent

active

047118474

ABSTRACT:
Secretin, which cannot be prepared directly by genetic engineering because of its carboxylic acid carboxyl-terminus, can be obtained by preparing secretylglycine by genetic engineering and then obtaining secretin therefrom by enzymatic conversion of the terminal glycine radical. The gene for the secretylglycine is synthesized chemically from smaller single-stranded units which are linked enzymatically to give the complete gene, incorporated into a suitable vector and amplified therein, after which the peptide is isolated directly or as a fusion protein and, after cyanogen bromide cleavage, is converted enzymatically into secretin.

REFERENCES:
Mutt et al., (1970), Eur. J. Biochem., vol. 15, pp. 513-519.
Shine et al., (1980), Nature, vol. 285, pp. 456-461.
Rossi et al., (1982), J. Biol. Chem., vol. 257, pp 9226-9229.
Suzuki et al., (1982), Proc. Nat'l Acad. Sci., U.S.A., vol. 79, pp. 2475-2479.
Bradbury, A. et al., 1982, Nature, 298: 686, 301.
Husain and Tate, FEBS Letters, 152: 277-281, (1983).

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