Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-12-22
1998-02-03
Walsh, Stephen
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
530350, 530412, C12N 1512, C07K 1447
Patent
active
057143471
ABSTRACT:
A method of preparing recombinant ubiquitin cross-reactive protein (UCRP) is disclosed. In one embodiment, the method comprises the step of purifying UCRP from a host cell in the presence of a cobalt or Group IIb metal, wherein the cobalt or Group IIb metal prevents proteolytic inactivation of UCRP occurring by cleavage of the UCRP carboxyl terminal glycine dipeptide. In another embodiment the method comprises purifying UCRP comprising an additional arginine or lysine residue at the carboxy terminal. A preparation of UCRP with an additional arginine residue at the carboxy terminus is also disclosed.
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Haas Arthur L.
Narasimhan Jana
Basham Daryl A.
MCW Research Foundation
Walsh Stephen
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