Preparation of recombinant Factor VIII in a protein free medium

Details Preparation of recombinant Factor VIII in a protein free medium Preparation of recombinant Factor VIII in a protein free medium

1997-04-18

1998-09-08

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435325, 435404, 530383, C12N 502, C12N 510, C12N 1512, C07K 14755

Patent

active

058044205

ABSTRACT:
Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolymers, preferably in the presence of trace metals such as copper. In very preferred embodiments, the medium includes a polyglycol known as Pluronic F-68, copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium.

REFERENCES:
patent: 5024947 (1991-06-01), Inlow
patent: 5378612 (1995-01-01), Nakashima

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