Preparation of reagent for immune complex isolation

Details Preparation of reagent for immune complex isolation Preparation of reagent for immune complex isolation

1986-01-09

1988-11-08

Nucker, Christine M.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

424101, 436506, 436507, 436509, 436542, 530419, 530829, 530830, A61K 3514, C07K 700

Patent

active

047835257

ABSTRACT:
To diagnose diseases in patients, a protein complex, RhC, is prepared from horse serum by precipitating a white powder from the serum at a pH of 5.5 and processing to remove lipids at a pH of 8.2 using Tris-HCl as the buffer. It includes two components associated together to provide a molecular weight of 280,000 and having characteristics of a rheumatoid factor and a Clq-like subcomponent of the complement. The protein complex is incubated with human serum or plasma and then precipitated by dialysis against a high pH buffer (0.05 M Tris-HCl pH 8.2). When precipitated, it co-precipitates the immune complexes from the human blood serum without substantial monomeric immunoglobulin to quantitatively isolate immune complexes from serum. Immunological assays then determine how much immune complex and what kind were in the serum.

REFERENCES:
patent: 4283383 (1981-08-01), Masson et al.
patent: 4420461 (1983-12-01), Reckel et al.
McDonald, Chem. Abstracts, 96 (1982) #197638a.

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