Preparation of pure cultures of post-mitotic human neurons

Details Preparation of pure cultures of post-mitotic human neurons Preparation of pure cultures of post-mitotic human neurons

1996-06-10

1997-08-05

Ketter, James

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

C12N 506, C12N 510

Patent

active

056541899

ABSTRACT:
NTera 2/cl.D1 (NT2) cells, a human teratocarcinoma cell line, were manipulated following retinoic acid (RA) treatment to yield >95% pure cultures of neuronal cells (NT2-N cells). This culture method is capable of yielding sufficient highly differentiated post-mitotic NT2-N cells for both biochemical and molecular biological studies. NT2 cells can be transfected efficiently and the transfected gene products can be expressed in both NT2 and NT2-N cells.

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