Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
1996-03-19
2002-06-25
Romeo, David S. (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S068100, C435S320100, C435S174000, C530S300000, C530S395000
Reexamination Certificate
active
06410269
ABSTRACT:
FIELD OF THE INVENTION
This invention relates to potent macrophage activating factors, prepared by oligosaccharide digestion of the cloned vitamin D binding protein (Gc protein) and the cloned Gc protein domain III, and the use of these macrophage activating factors for various cancer, HIV-infection and osteopetrosis, and as adjuvants for immunization and vaccination.
TABLE OF TERMS
Gc protein
Vitamin D
3
-binding protein
MAF
macrophage activating factor
GcMAF
Gc protein-derived macrophage activating protein
GcMAFc
cloned Gc protein-derived macrophage activating factor
Gc domain III
domain III region of Gc protein
CdMAF
cloned domain III-derived macrophage activating factor
SUMMARY OF THE INVENTION
Vitamin D-binding protein (Gc protein) and its small domain (approximately ⅕ of the Gc peptide also known as domain III) were cloned via a baculovirus vector. The cloned Gc protein and the cloned domain (Cd) peptide were treated with immobilized &bgr;-galactosidase and sialidase to yield macrophage activating factors, GcMAFc and CdMAF, respectively. These cloned macrophage activating factors and GcMAF are to be used for therapy of cancer, HIV-infection and osteopetrosis, and may also be used as adjuvants for immunization and vaccination.
REFERENCES:
patent: 5177001 (1993-01-01), Yamamoto
patent: 5177002 (1993-01-01), Yamamoto
patent: 5326749 (1994-07-01), Yamamoto
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Caesar Rivise Bernstein Cohen & Pokotilow Ltd.
Romeo David S.
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