Preparation of plasmid DNA and products thereof

Details Preparation of plasmid DNA and products thereof Preparation of plasmid DNA and products thereof

1984-04-23

1988-07-05

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435173, 435253, 435320, 935 19, 935 42, C12P 2100, C12N 1500, C12N 100, C12N 120

Patent

active

047554649

ABSTRACT:
A method for the preparation of plasmid DNA and the expression products thereof. The method provides cells, enriched in plasmid DNA and expression products thereof, which comprise chromosomal DNA which has been substantially degraded by exposing a plasmid DNA-containing organism to an effective dose of ionizing radiation.

REFERENCES:
patent: 4311797 (1982-01-01), Khachatourians
Sancar; A., A. M. Hack and W. D. Rupp, J. Bacteriology 137(1): 692-693, 1979.
Kapp; D. S. and K. C. Smith, J. Bacteriology 103(1):49-54 1970.
A. J. Radford, D. G. MacPhee, and D. C. Reanney, Plasmid, 10, 299-302(1983).
R. W. Tuveson et al., Photochemistry and Photobiology, 37, 109(1983).
M. H. L. Green et al., J. of General Microbiology, 67, 63(1971).
R. M. Tyrrell, Int. J. Radiat. Biol., 25, 373(1974).

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