Preparation of lysocellin beads by fermentation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

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435119, 435244, 549414, C12P 1716, C12P 1718

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active

058495509

ABSTRACT:
A method for producing lysocellin in bead form comprises:
(a) cultivating a lysocellin-producing microorganism in an aqueous nutrient-containing fermentation broth under lysocellin-producing conditions;
(b) adding a base or inorganic phosphate to the fermentation broth within about the first 24 hours of the beginning of the fermentation in an amount sufficient to ensure that the lysocellin is produced in the form of a cation salt of lysocellin;
(c) providing a lipid to the fermentation broth such that the lipid is present in the broth in a sufficient amount to form discrete beads with the lysocellin in the fermentation broth; and
(d) separating the beads from the broth.

REFERENCES:
patent: 4033823 (1977-07-01), Liu et al.
patent: 4478935 (1984-10-01), Williams et al.
patent: 5041374 (1991-08-01), Chu et al.
patent: 5047338 (1991-09-01), Miescher
patent: 5047339 (1991-09-01), Mehrotra

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