Preparation of ligand-polymer conjugate having a controlled numb

Details Preparation of ligand-polymer conjugate having a controlled numb Preparation of ligand-polymer conjugate having a controlled numb

1987-11-09

1990-11-06

Nutter, Nathan M.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

525 542, 5253274, 5253294, 5253295, 530402, 530403, 530404, 530405, 530406, C08F 2008, C08G 6348, C08G 6391, C08L 8900

Patent

active

049687425

ABSTRACT:
A method for chemically coupling a controllable number of ligands, e.g., haptens, to a polymeric material by derivatization of the polymer with an activating agent to introduce a couplable functional group. The derivatization is performed in the presence of a blocking agent which is reactive with the same functionality on the polymer as the activating agent. By reacting the polymer with a mixture of a predetermined ratio of excess amounts of the activating and blocking agents, a controllable number of couplable functional groups are introduced to the polymer for subsequent linkage to the desired ligand. The resulting polymer-ligand conjugates are useful as reagents in immunoassays, particularly immunoturbidimetric assays.

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Makromol. Chem., Rapid Commun. 8, 431-435, (1987), Yokoyama et al., "Preparation of Adriamycin-Conjugated Poly(Ethylene Glycol)-Poly(Aspartic Acid) Block Copolymer".
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Anderson et al., "The Use of Esters of N-Hydroxysuccinimide in Peptide Synthesis", J. Am. Chem. Soc., 86, May 5, 1964, pp. 1839-1842.
Sutoh et al., "Electron Microscopic Visualization of the SH, Thiol of Myosin by the Use of an Avidin-Biotin System", J. Mol. Biol., (1984), 178, 323-339.

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