Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1996-07-02
1998-09-15
Naff, David M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
4242041, 4242081, 4242121, 4242151, 4242171, 4242191, 4242261, 4242271, 4242291, 4242451, 4242471, 4242491, 4242861, 424 931, 424 934, 424 9341, 435182, 42840224, 530817, G01N 33544, A01N 6300, C12N 1104
Patent
active
058077570
ABSTRACT:
A method is provided for preparing polyphosphazene microspheres wherein the polyphosphazene microspheres are produced by coacervation. A solution containing a polyphosphazene is admixed with a solution containing a salt of a monovalent ion such as a salt of a Group I element (for example, NaCl) to form a dispersion containing polyphosphazene coacervate microdroplets. The dispersion then is admixed with a solution containing a salt of a multivalent ion, such as a salt of a Group II element (for example, CaCl.sub.2) to form a suspension of polyphosphazene microspheres. The polyphosphazene microspheres then are recovered from the suspension. Such method enables one to obtain high yields of microspheres having a controlled size distribution. Polyphosphazene microspheres containing biological material can be produced by providing a biological material in the polyphosphazene solution that is mixed with the solution containing a salt of a monovalent ion. The biological material may be an antigen or other biological material selected from proteins, nucleic acids, polysaccharides and synthetic compounds having biological activity.
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Andrianov Alexander K.
Chen Jianping
Lillie Raymond J.
Naff David M.
Olstein Elliot M.
Virus Research Institute, Inc.
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