Preparation of immobilized enzymatically-active substance

Details Preparation of immobilized enzymatically-active substance Preparation of immobilized enzymatically-active substance

1980-02-25

1982-06-08

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 435182, C12N 1110, C12N 1108, C12N 1104

Patent

active

043340270

ABSTRACT:
Immobilized enzymatically-active substance is prepared by forming a combination of an enzymatically-active substance and an aqueous polyelectrolyte solution containing a substantial amount of alginate, adding the combination to an aqueous precipitation both containing a source of multivalent ion to form beads of gel containing the enzymatically-active substance, drying the gel beads under controlled conditions to significantly reduce the diameter of the beads and cause hardening of the beads to provide increased mechanical strength, and adding the dried beads to a precipitation both containing a multivalent ion to effect further hardening of the beads. The combination of enzymatically active substance and polyelectrolyte solution may contain an epoxy prepolymer. After further hardening in the polyelectrolyte solution, the beads may be suspended in a buffer solution to dissolve the polyelectrolyte and form porous beads. The process enables manufacture of enzymatically active beads of great mechanical strength, having a high content of enzymatic substance and a high degree of porosity.

REFERENCES:
patent: 3733205 (1973-05-01), Shovers et al.
patent: 4208482 (1980-06-01), Ehrenthal et al.
patent: 4259445 (1981-03-01), Glass et al.
Kierstan et al., The Immobilization of Microbial Cells, Subcellular Organelles and Enzymes in Calcium Alginate Gels, Biotech. & Bioeng., vol. XIX, 1977, (pp. 387-397).

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