Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2007-03-20
2007-03-20
Devi, S. (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S416000, C530S417000, C530S810000, C530S811000, C436S538000, C436S532000
Reexamination Certificate
active
10070764
ABSTRACT:
Toxin derivatives are prepared by proteolytic treatment of holotoxin, and their toxicity is reduced by contacting the preparation with a ligand, which can be a metal or an antibody or another ligand. This ligand selectively binds to the toxin but not to the toxin derivative. Removing the ligand and toxin bound to the ligand further reduces toxicity. A second ligand is used to remove conjugates of the toxin and the first ligand. Compositions contain the purified derivative, optionally plus the toxin and the ligand.
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Hallis et al. In: Botulinum and Tetanus Neurotoxins. (Ed) DasGupta et al. Plenum Press, New York, pp. 433-436, 1993.
Gimenez, J.A. and DasGupta, B.R., “Botulinum Type A Neurotoxin Digested with Pepsin Yields 132, 97, 72, 45, 42, and 18 kD Fragments,”J. Pro. Chem. 12:351-363, Plenum Publishing Corporation (1993).
Schiavo, G. et al., “Botulinum Neurotoxins Are Zinc Proteins,”J. Biol. Chem. 267:23479-23483, The American Society for Biochemistry and Molecular Biology, Inc. (1992).
Derwent Patent Abstract. File No. 351, Accession No. 9065531, English language abstract of DE 4037809A.
Alexander Frances Celine Gail
Chaddock John Andrew
Foster Keith Alan
Devi S.
Sterne Kessler Goldstein & Fox P.L.L.C.
The Health Protection Agency
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