Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1997-04-25
1999-09-07
Fox, David T.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
435 691, 435 692, 435 6952, 435 696, 435 697, 435 698, 435 701, 435 711, 435183, 435214, 435219, 435 694, 4352542, 43525421, 4353201, 435477, 536 232, 536 234, 536 2352, 536 236, 536 241, C12N 1581, C12N 1529, C12N 1562, C12N 1512
Patent
active
059486821
ABSTRACT:
The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.
REFERENCES:
Radke et al., "Transformation of Brassica napus L. using Agrobacterium tumefaciens: Developmentally Regulated Expression of a Reintroduced Napin Gene", Theor. Appln. Genet. (1988) 75:685-694.
Taylor et al., "Storage-protein Regulation and Lipid Accumulation in Microspore embryos of Brassica napus L.", Planta (1990) 181:18-26.
Sijmons et al., "Production of Correctly Processed Human Serum Albumin in Transgenic Plants" Bio/Technology (1990) 8:217-221.
Huang, "Lipid Bodies" Modern Methods Plant Analysis (1985) 1:145-151.
Misra and Gedamu, "Heavy Metal Tolerant Transgenic Brassica napus L. and Nicotiana tabacum L. Plants" Theor. Appl. Genet. (1989) 78:161-168.
Hatzopoulos et al., "Interaction of Nuclear Factors with Upstream Sequences of Lipid Body Membrane Protein Gene from Carrot" The Plant Cell (1990) 2:457-467.
Lee et al., "Maize Oleosin is Correctly Targeted to Seed Oil Bodies in Brassica napus Transformed with the Maize Oleosin Gene" PNAS USA (1991) 88:6181-6185.
Vance and Huang, "Expression of Lipid Body Protein Gene during Maize Seed Development" J. Biol. Chem. (1988) 263:1476-1481.
Vance and Huang, "The Major Protein from Lipid Bodies of Maize" J. Biol. Chem. (1987) 262:11275-11279.
Qu and Huang, "Oleosin KD 18 on the Surface of Oil Bodies in Maize" J. Biol. Chem (1990) 265:2238-2243.
Sengupta-Gopalan et al., "Developmentally Regulated Expression of the Bean Beta-phaseolin Gene in Tobacco Seed" PNAS USA (1985) 82:3320-3324.
Fraley et al., "Expression of Bacterial Genes in Plant Cells" PNAS USA (1983) 80:4803-4807.
Vanderkerckhove et al., "Enkephalins Produced in transgenic Plants using Modified 2S Seed Storage Proteins" BIO/Technology (1989) 7:929-932.
Murphy et al., "Synthesis of the Major Oil-body Membrane Protein in Developing Rapeseed (Brassica napus) Embryos" Biochem. J. (1989) 258:285-293.
Qu et al., "Characteristics and Biosynthesis of Membrane Proteins of Lipid Bodies in the Scutella of Maize (Zea mays L.)" Biochem. J. (1986) 235:57-65.
Josefsson et al., "Structure of a Gene Encoding the 1.7 S Storage Protein Napin, from Brassica napus" J. Biol. Chem (1987) 262:12196-12201.
Scofield and Crouch, "Nucleotide Sequence of A Member of the Napin Storage Protein Family From Brassica napus" J. Biol. Chem. (1987) 262:12202-12208.
Fujikawa et al., "Bovine Factor X1 (Stuart Factor), Mechanism of Activation by a Protein from Russell's Viper Venom" Biochemistry (1972) 11:4892-4899.
Nagai et al., "Oxygen Binding Properties of Human Mutant Hemoglobins Synthesized in Escherichia coli" PNAS USA (1985) 82:7252-7255.
Scholtissek and Grosse, "A Plasmid Vector System for the Expression of a Triprotein Consisting of Beta-galactosidase, a Collagenase Recognition Site and a Foreign Gene Product" Gene (1988) 62:55-64.
Bevan, "Binary Agrobacterium Vectors for Plant Transformation" Nucl. Acids. Res. (1984) 12:8711-8721.
Murphy et al., "A class of Amphipathic Proteins Associated with Lipid Storage Bodies in Plants" Biochem. Biophys. Acta (1991) 1088:86-94.
Antoni et al., "A Short Synthetic Peptide Fragment of Human Interleukin 1 with Immunostimulatory But not Inflammatory Activity" J. Immunol. (1986) 137:3201-3204.
An et al., "New Cloning Vehicles for Transformation of Higher Plants" Embo J. (1985) 4:277-284.
Hood et al., "The Hypervirulence of Agrobacterium tumefaciens A281 is encoded in a Region of pTiBo542 outside of T-DNA" J. Bacteriol. (1986) 168:1291-1301.
Holbrook et al., "Oilbody Proteins in Microspore-derived Embryos of Brassica napus" Plant Physiol. (1991) 97:1051-1058.
Kalinski et al., "Molecular Cloning of a Protein Associated with Soybean Seed Oil Bodies that is Similar to Thiol Proteases of the Papain Family" J. Biol. Chem. (1990) 265:13843-13848.
Fox David T.
Sembiosys Genetics Inc.
LandOfFree
Preparation of heterologous proteins on oil bodies does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Preparation of heterologous proteins on oil bodies, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Preparation of heterologous proteins on oil bodies will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1804037