Preparation of gel-filled separation columns

Details Preparation of gel-filled separation columns Preparation of gel-filled separation columns

1990-06-14

1991-10-29

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2041806, 264 22, C25B 100, C25B 700, B01D 6142

Patent

active

050613553

ABSTRACT:
A method of preparing a microcapillary gel separation column for electrophoresis comprises: pretreatment of a tube with a bifunctional reagent to provide polymer bonding sites on the inner wall of the tube; filling the tube with a monomer solution a crosslinking agent, and a photoinitiator; forming a polymer plug by illuminating an initiation section of the tube; growing the plug by moving the tube gradually past the illumination source; and stopping tube motion when the desired length of gel is attained. Provision is made for monitoring polymerization and adjusting tube movement speed to avoid gaps in the growing polymer.

REFERENCES:
patent: 4790919 (1988-12-01), Baylor
patent: 4810456 (1989-03-01), Bente, II et al.
patent: 4865706 (1989-09-01), Karger et al.
patent: 4865707 (1989-09-01), Karger et al.

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