Preparation of gel-filled separation columns

Details Preparation of gel-filled separation columns Preparation of gel-filled separation columns

1991-06-28

1993-04-06

Niebling, John

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Automatic analytical monitor and control of industrial process

422105, 422107, 204200R, G01N 2100, G01N 3100, G01N 3300

Patent

active

052001504

ABSTRACT:
A method of preparing a microcapillary gel separation column for electrophoresis comprises: pretreatment of a tube with a bifunctional reagent to provide polymer bonding sites on the inner wall of the tube; filling the tube with a monomer solution a cross-linking agent, and a photoinitiator; forming a polymer plug by illuminating an initiation section of the tube; growing the plug by moving the tube gradually past the illumination source; and stopping tube motion when the desired length of gal is attained. Provision is made for monitoring polymerization and adjusting tube movement speed to avoid gaps in the growing polymer.

REFERENCES:
patent: 4572819 (1986-02-01), Priddy et al.
patent: 4619901 (1986-10-01), Webb et al.
patent: 4704198 (1987-11-01), Ebersole et al.
patent: 4790919 (1988-12-01), Baylor, Jr.
patent: 4997537 (1991-03-01), Karger et al.

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