Preparation of cross-linked glucose isomerase crystals

Details Preparation of cross-linked glucose isomerase crystals Preparation of cross-linked glucose isomerase crystals

1993-11-08

1995-08-01

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435174, 435 94, C12N 992, C12N 1100, C12P 1924

Patent

active

054379938

ABSTRACT:
The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst. Preferrably the compound having an amino group is an ammonium salt, lysine or tryptophan and the dialdehyde is glutaraldehyde.

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Lee et al., Bioorganic Chemistry, vol. 14, 202-210, 1986.
Quiocho et al., NAS Proceedings, vol. 52, 1964.
Bachman et al., Starch/Starke, 33, Nr. 2, 1981.

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