Preparation of contaminant free containers of culture medium

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435 31, 435297, 435299, 435801, 435810, 435800, C12N 120

Patent

active

042620910

ABSTRACT:
A method and apparatus are described for providing petri dishes of a culture medium in an oxygen-free manner. Empty petri dishes are supplied to a dispensing glove box chamber providing isolation from the ambient atmosphere, by communicably connecting to such dispensing chamber, another glove box chamber providing isolation from the ambient atmosphere containing the empty dishes. The dishes are sequentially transferred to the dispensing chamber where they are filled with autoclaved culture medium. The filled dishes are transferred to a third chamber (also communicably connected to the dispensing chamber) within which the culture medium can solidify. Once the third chamber is filled with dishes having culture medium it is disconnected from the dispensing chamber and replaced with a fourth chamber having a new batch of unfilled petri dishes. The dishes from the new batch are then transferred into the dispensing glove box chamber and, once filled, transferred into the now empty chamber which provided the original batch of unfilled dishes. Each chamber containing filled petri dishes maintains such dishes in an isolated environment for a selected incubation period, and the dishes therein are then individually packaged in hermetically tight packages prior to being exposed to the ambient atmosphere.

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patent: 4111753 (1978-09-01), Folsom et al.

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