Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1999-12-20
2000-10-31
Ketter, James
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
4353201, 536 231, C12N 1564
Patent
active
061401285
ABSTRACT:
A process is described for preparing transfectacons, or particles, of calcium phosphate and a desired nucleic acid comprising admixing calcium divalent cation, phosphate multivalent anion, and the desired nucleic acid to form a precipitation mixture, wherein the precipitation mixture comprises an initial phosphate anion concentration of about 0.2 to 0.5 mM; and incubating the precipitation mixture for about 10 to 60 minutes to form transfectacons comprising calcium phosphate and the desired nucleic acid. A process is also provided for delivering desired nucleic acid to eukaryotic tissue or cells comprising introducing to the tissue or cells the transfectacons so prepared. Additionally, a process is disclosed for introducing a desired nucleic acid into a eukaryotic host cell comprising the above two steps, followed by diluting the precipitation mixture and admixing it with a eukaryotic host cell lacking a cell wall to form a transfection mixture; and incubating the transfection mixture to allow the eukaryotic host cell to take up the transfectacons to form a transfected cell.
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Cohen Darien L.
Kahn David W.
Winkler Marjorie E.
Genentech Inc.
Hasak Janet E.
Ketter James
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