Preparation of biosensor having a layer containing an enzyme, el

Details Preparation of biosensor having a layer containing an enzyme, el Preparation of biosensor having a layer containing an enzyme, el

1990-11-26

1993-07-20

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 4, 435 14, 435176, 435179, 435182, 435817, 435288, 204403, C12N 1102, C12N 1112, C12N 1104, C12M 140

Patent

active

052292827

ABSTRACT:
The present invention relates to a process of preparation of a biosensor comprising forming an electrode system mainly containing carbon on an insulating base plate, treating the surface of electrode system with an organic solvent, and then arranging a reaction layer on the electrode system to give a unified element. The reaction layer contains an enzyme, electron acceptor and a hydrophilic polymer. Treatment with the organic solvent improves adhesion of the reaction layer to the electrode system. The electrode system contains a working electrode and a counter electrode. The electrode system is formed from a carbon paste containing a resin binder. Treatment is preferably carried out by wiping the surface of the electrodes with a material impregnated by the organic solvent.

REFERENCES:
patent: 4418148 (1983-11-01), Oberhardt
patent: 4897173 (1990-01-01), Nankai et al.
Miyamaki, et al., Biochimica et Biophysica Acta, vol. 838, 1985, pp. 60-68.

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