Preparation of antiserum for quantitative determination of X and

Details Preparation of antiserum for quantitative determination of X and Preparation of antiserum for quantitative determination of X and

1976-07-16

1979-04-03

Fagelson, Anna P.

Drug, bio-affecting and body treating compositions

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Taste or smell or chemical irritation to the eye, nose, or...

23230B, 1951035A, 260112R, 424 85, 424 88, 424101, 424177, A23J 106, G01N 3102, G01N 3316

Patent

active

041477650

ABSTRACT:
An antiserum for the quantitative determination of X and Y degradation products of fibrin and fibrinogen is produced by administering the purified degradation products to an experimental animal thereby to immunize said animal, drawing serum from said animal, contacting said serum with a plasma and separating the resultant precipitate, thereby to leave a supernatant liquid which constitutes said antiserum. The plasma may be a heparin, citrate, oxalate, ethylenediaminetetracetate or glutaraldehyde-treated plasma and as many as 12 or 15 precipitations may be made with the animal antiserum. The antiserum may be fractionated at any stage by adsorption on Sephadex modified dextran.

REFERENCES:
Marder, J. Biol. Chem., vol. 244, 1966, pp. 2111-2119.
Fisher, J. Lab. & Clin. Med., vol. 70, 1967, pp. 903-922.

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