Preparation of an insoluble biocatalyst

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435180, 530817, C12N 1104, C12N 1108, C07K 1708

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047644672

ABSTRACT:
An insoluble biocatalyst is prepared by forming a mixture consisting essentially of the biocatalyst, one or more water-soluble compounds having two or more acrylamide or meth-acrylamido groups and one or more water soluble amines possessing two or more hydrogen atoms bonded to an amine nitrogen, and forming a gel from the mixture. The water-soluble amine is selected from ammonia, lower diamines, lower polyamines, primary monoamines, polyethyleneimine and polyvinylamine. Beads containing the biocatalyst may be formed by suspending the mixture in a water-immiscible solvent to form droplets prior to gelling. The biocatalyst may be an enzyme, cells or cell fragments.

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Nilsson et al., Biochimica et Biophysica Acta, vol. 268, 1972, pp. 253-256.

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