Preparation of an enzyme-cellulose complex

Details Preparation of an enzyme-cellulose complex Preparation of an enzyme-cellulose complex

1974-10-08

1976-06-01

Naff, David M.

Textiles: fiber preparation

195 63, 195DIG11, 426 36, A23C 1902, C07G 702

Patent

active

039606660

ABSTRACT:
Enzymes are fixed to cellulose containing 5 to 25% lignin to form an enzyme-cellulose complex by a process involving treating cellulose pieces with an alkaline solution to remove lignin on the surface of the pieces, treating the resultant cellulose pieces with a sulfochlorinating or a chlorinating agent in an excess of pyridine until sulfur and/or chlorine is combined with the cellulose, washing the sulfur and/or chlorine-containing cellulose pieces and treating the pieces with an aqueous enzyme solution to attach enzymes thereto. A very important application is preparation of a rennet-cellulose complex and using the complex for coagulating milk in making cheese.

REFERENCES:
patent: 3841969 (1974-10-01), Emery et al.
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, May 1973 (pp. 28 and 35)
Weliky et al., The Chemistry and Use of Cellulose Derivatives for the Study of Biological Systems, Immunochemistry, vol. 2, 1965 (pp. 293-303).

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