Preparation of an Auricularia auricula polysaccharide and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C514S054000, C536S123000, C536S123100, C536S123120, C536S127000

Reexamination Certificate

active

07029883

ABSTRACT:
A method for producing a purifiedAuricularia auriculaPolysaccharide (AP) extract ofAuricularia auricula(AA). A hydrated predetermined amount of AA is filtered and extracted, producing a first pre-extract. A plurality of additional hydrated predetermined amounts of AA is separately filtered and extracted to produce a plurality of additional pre-extracts. The pre-extracts are combined to form a first extract. The first extract is concentrated and suspended, forming a precipitate. The precipitate is purified to form a first filtrate, and repurified to form a second filtrate. The first and second filtrates are combined to form a first combined filtrate, which is concentrated, purified and dried to form a concentrated extract. The concentrated extract is purified to form a third filtrate, and repurified to form a fourth filtrate. The third and fourth filtrates are combined to obtain a second combined filtrate, which is concentrated, purified and dried to obtain the purifiedAuricularia auriculaPolysaccharide extract of AA. A method of reducing the serum cholesterol in mammals by administering a pharmaceutically effective amount of the purifiedAuricularia auriculaPolysaccharide (AP) extract ofAuricularia auricula(AA) produced according to the foregoing method.

REFERENCES:
patent: 59-163323 (1984-09-01), None
Zhang et al, Carbohydrate Res. 270:1-10, 1995.
Kiho et al, Chem. Pharm. Bull. 39(3):798-800, 1991.
Kiho et al, Carbohydrate Res. 142:344-351, 1985.
Misaki et al, Carbohydrate Res. 92:115-129, 1981.
Sone et al, Agric. Biol. Chem. 42(2):417-425, 1978.

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