Preparation of an acylated protein powder

Details Preparation of an acylated protein powder Preparation of an acylated protein powder

1995-06-07

1997-12-23

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

530344, 530345, 530410, 530419, C07K 130, C07K 136, C07K 1462

Patent

active

057009040

ABSTRACT:
A method for recovering an acylated protein as a powder, especially in cases where said acylated protein is one that resists isolation by isoelectric precipitation from aqueous solutions of the protein, said method comprising in combination adjusting said aqueous solution to near the isoelectric pH of the protein and providing a suitable alcohol concentration to cause precipitation of the protein in the form of filterable particles at said pH.

REFERENCES:
patent: 3471464 (1969-10-01), Bellet
patent: 3528960 (1970-09-01), Haas
patent: 3591574 (1971-07-01), Fenichel
patent: 3752798 (1973-08-01), Amird
patent: 3755569 (1973-08-01), Fenichel
patent: 3823125 (1974-07-01), Grant
patent: 3843815 (1974-10-01), Reesman
patent: 3864325 (1975-02-01), Smyth
patent: 3868356 (1975-02-01), Smyth
patent: 3868357 (1975-02-01), Smyth et al.
patent: 3869437 (1975-03-01), Lindsay
patent: 3883496 (1975-05-01), Geiger
patent: 3883500 (1975-05-01), Geiger et al.
patent: 3884897 (1975-05-01), Geiger et al.
patent: 3950517 (1976-04-01), Lindsay et al.
patent: 4013628 (1977-03-01), Obermeier
patent: 4014861 (1977-03-01), Geiger et al.
patent: 4486458 (1984-12-01), Bradford et al.
patent: 5304473 (1994-04-01), Belagaje
Anderson, et al., "The Use of Esters of N-Hydroxysuccinimide in Peptide Synthesis," Journal of American Chemical Society, 86:1839-1842 (1964).
Asada, et al., "Stability of Acyl Derivatives of Insulin in the Small Intestine: Relative Importance of Insulin Association Characteristics in Aqueous Solution," Pharmaceutical Research, 11(8):1115-1120 (1994).
Geiger, et al., "Contribution of Peptide Chemistry to our Knowledge of Insulin and Diabetes," from Proceedings of the Symposium on Proinsulin, Insulin and C-Peptide, Tokushima, Jul. 12-14, pp. 62-72 (1978).
Geiger, et al, "Biological Activity of Insulin Analogues Substituted at the Amino Group of B1-Phenylalanine", from Proceedings of the Second International Insulin Symposium, Aachen, Germany, Sep. 4-7, pp. 409-415, (1979).
Geiger, "Chemie des Insulins," Sonderdruck 100:111-129, (1976) (Translation Attached).
Hashimoto, et al., "Synthesis of Palmitoyl Derivatives of Insulin and Their Biological Activities," Pharmaceutical Research, 6(2):171-176 (1989).
Hashizume, et al., "Improvement of Large Intestinal Absorption of Insulin by Chemical Modification with Palmitic Acid in Rats," J. Pharm. Pharmacol., 44:555-559 (1992).
Inoue, et al., "Synthesis of a Superoxide Dismutase Derivative That Circulates Bound to Albumin and Accumulates in Tissues Whose pH is Decreased," Biochemistry, 28(16):6619-6624 (1989).
Kunitomo, et al., "Synthesis of Cytochrome c Derivative with Prolonged In Vivo Half-life and Determination of Ascorbyl Radicals in the Circulation of the Rat," The Journal of Biological Chemistry, 267(13):8732-8738 (1992).
Lapidot et al., "Use of Esters of N-hydroxysuccinimide in the Synthesis of N-acylamino Acids," Journal of Lipid Research, 11(8):1115-1120 (1994).
Lindsay et al., "Acetoacetylation of Insulin," Biochem. J., 115:587-595 (1969).
Lindsay et al., "The Acetylation of Insulin," Biochem. J., 121:737-745 (1971).
MacIntyre et al., "Information About Insulin by Chemical and Enzymatic Modifications," Molecular Endocrinology, Proceedings of Endocrinology '77 held at the Royal College of Physicians, London, England Jul. 11-15, pp. 27-42 (1977).
Muranishi, et al., "Trials of Lipid Modification of Peptide Hormones for Intestinal Delivery," Journal of Controlled Release, 19:179-188 (1992).
Riordan, et al., "Acetylation," Methods of Enzymology,25:494-499 (1972).
Roosen et al., "A1-Modified Insulins: Receptor Binding and Biological Activity," Insulin Chemistry, Structure and Function of Insulin and Related Hormones from Proceedings of the Second International Insulin Symposium, Aachen, Germany, Sep. 4-7, pp. 403-408 (1979).
Scheider, "Ligand-Independent Activated State of Serum Albumin for Fatty Acid Binding," Journal of Physical Chemistry, 84(8):925-928 (1980).
Harris, E. L. V. and Angal, S. Protein Purification Methods: a Practical Approach. Oxford: Oxford University Press. 1989, pp. 152-160.

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