Preparation of a solid phase matrix containing a bound specific

Details Preparation of a solid phase matrix containing a bound specific Preparation of a solid phase matrix containing a bound specific

1991-12-23

1993-12-07

Naff, David M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

435 71, 435 792, 435 793, 435 794, 435176, 435177, 435178, 435180, 435181, 436524, 436528, 436529, 436531, 436532, 530811, 530812, 530813, 530815, 530816, G01N 33552, G01N 3353, C12N 1114, C12N 1102

Patent

active

052683060

ABSTRACT:
A specific binding pair is bound to an insoluble carrier for use in determining an analyte such as in an immunoassay. The carrier is coated with a first polymer containing a protein polymer having a molecular weight of at least about 20,000 and molecules of a first member of a specific binding pair. A second polymer containing a second member of the specific binding pair is bound to the first member on the carrier by binding of the first and second members of the specific binding pair. The first polymer is preferably more hydrophobic than the second polymer. The protein polymer can be prepared by cross-linking hydrophobic protein molecules of 10,000 to 700,000 molecular weight with a bifunctional or polyfunctional compound to obtain a protein polymer of 200,000 to 20,000,000 molecular weight. The second polymer can be the second member of the specific binding pair or the second member cross-linked with a linker or the second member cross-linked to a hydrophobic protein.

REFERENCES:
patent: 4282287 (1981-08-01), Giese
patent: 4778751 (1988-10-01), El Shami
patent: 4820644 (1989-04-01), Schafer
patent: 5061640 (1991-10-01), Tischer et al.

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