Preparation of a selectively permeable membrane for an enzyme el

Details Preparation of a selectively permeable membrane for an enzyme el Preparation of a selectively permeable membrane for an enzyme el

1989-01-24

1991-07-23

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

204403, 435180, 435182, 435817, C12M 140, C12N 1108, C12N 1104, G01N 2726

Patent

active

050343300

ABSTRACT:
A selectively permeable membrane for an enzyme electrode is prepared by forming a mixture of an emulsion of resin containing at least one prepolymer or monomer having ethylenic unsaturation, and a water-soluble polymer material, and subjecting the mixture to irradiation with ionizing or ultraviolet irradiation. The membrane is permeable to substances produced by an enzyme reaction while preventing the permeation of higher molecular weight materials. An immobilized enzyme membrane is formed on an outer surface of the permeable membrane, and the resultant membrane is mounted on an electrically conductive base to form an enzyme electrode. In another embodiment, the selectively permeable membrane is formed on the electrically conductive base, and then the immobilized enzyme membrane is formed. The selectively permeable membrane is situated between the immobilized enzyme membrane, which is in contact with a sample solution, and the electrically conductive base.

REFERENCES:
patent: 3860490 (1975-01-01), Guttag
patent: 4356074 (1982-10-01), Johnson
patent: 4795707 (1989-01-01), Niiyama et al.
Y. Hanazato et al.: "Application of Water-Soluble Photocrosslinkable Polymer to Enzyme Membrane for Fet-Biosensor," Proc. of 2nd International Meeting on Chemical Sensors, Bordeaux, Jul. 7-11, 1986, pp. 576-579.
I. Takatsu et al., "Solid State Biosensors Using Thin-Film Electrodes", Sensors and Activators, vol. 11, 1987, pp. 309-317.

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