Preparation of a radioconjugate formulation

Details Preparation of a radioconjugate formulation Preparation of a radioconjugate formulation

1994-11-21

1998-04-28

Kight, John

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Attached to antibody or antibody fragment or immunoglobulin;...

424 153, 424 169, A61K 5108, A61K 5110

Patent

active

057441197

ABSTRACT:
The present invention is directed to a method of labeling a protein conjugate with a radionuclide to form a radioconjugate comprising reacting:
(a) the radionuclide; and
(b) the protein conjugate in an acetate buffer for a time period and under conditions sufficient to form said radioconjugate.
Preferably, the protein conjugate is comprised of a complexing agent moiety and a free epsilon amino group-containing protein moiety. The completing agent moiety is preferably a terpyridine analog. The terpyridine analog is preferably TMT.
Preferably, the free epsilon amino group-containing protein moiety is an immunoreactive protein. The immunoreactive protein is preferably the monoclonal antibody ING-1.
In a preferred embodiment, the protein conjugate is TMT-ING-1, and the radionuclide is .sup.90 Y.
In a further preferred embodiment, the radioconjugate is chelated to greater than 98 percent of the radionuclide.
Preferably, the acetate buffer in which the protein conjugate is present comprises at least about 50 mM sodium acetate.

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patent: 4885363 (1989-12-01), Tweedle et al.
patent: 5128119 (1992-07-01), Griffiths
patent: 5328679 (1994-07-01), Hansen et al.
patent: 5549882 (1996-08-01), Subramanian
Medline #91338631, Robinson et al.
Medline #90352555, Frank et al.

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