Preparation of a novel NADP linked alcohol-aldehyde/ketone oxido

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435 25, 435 26, 435190, 435814, 435822, 435842, C12N 902, C12N 904, C12Q 132

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043528856

ABSTRACT:
A partially purified NADP specific thermostable alcohol, aldehyde/ketone oxidoreductase is prepared which can react with a wide range of alcohols, ketones and aldehydes. The enzyme has a unique preference for secondary over primary alcohols. The thermostability, broad range of operating temperatures and lack of sensitivity to metal ions and complexing agents, in addition to the absolute specifity for the coenzyme, increase the utility of the enzyme in asymmetric organic synthesis and NADPH regeneration.

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Hou et al., Applied and Environmental Microbiology, Jul. 1979, pp. 135-142, vol. 38, No. 1.
Patel et al., Eur. J. Biochem. 101, 401-406 (1979).
Barth et al., Zeitschrift fur Allgemeine Mikrobiologie, 19, 381-390 (1979).
Zeikus et al., Enzyme Microb. Technol., vol. 1, pp. 243-252 (Oct. 1979).

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